Testing for Simple Sugars - The Benedict's Test

Aim: To test a sample of food to determine whether it contains simple sugars.

Equipment: Bunsen burner, test tube tongs, Benedict's Solution, a sample of food.

Method:
1.Place about 2mL of the sample in a test tube.
2. Add 3-5 drops of iodine and mix.


Results: If complex sugars (i.e. starch) are present the iodine solution will change from a brown color to a blue/black color. If the iodine solution does not change color then no complex sugars are present.









When the benedict solution was added into the orange juice and heat was applied the orange color went from orange to green because the Benedicts turned from blue to red-brown but if you mix it with orange it turns into green.

Conclusion: It changed color because simple sugars were present.