Making a Dilution Series

Aim: To make a dilution series to investigate concentration.

Equipment: A potassium permanganate crystal, six large tes tubes, tweezers, a plastic transfer pipette, a test tube rack, 10 mL measuring cylinder.

Method: 1. Place the six test tubes in a test tube rack. Label the rack with numbers 1-6.
2. using the measuring cylinder, fill the test tube 1 with 10 ml of water. Fill the remaining test tubes with 5ml of water
3. Using your tweezers, add a single crystal of potassium permanganate to test tube 1.
4. Gently shake the test tube until the crystal has dissolved.
5. Using the transfer pipette, carefully remove exactly 5 ml from test tube 1 and pour it into test tube 2.
6. Rinse the transfer pipette thoroughly to ensure that no purple solution remains.
7. Gently shake test tube 2 and repeat the transfer process, transferring exactly 5 ml of solution from test tube 2 to test tube 3.
8. Rinse the pipette again and repeat the transfer process for test tubes 4, 5 and 6.

Results: Our experiment went well and the water went lighter and lighter. Our experiment was a success.